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peroxidase conjugated goat anti human igg fab2  (Vector Laboratories)


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    Structured Review

    Vector Laboratories peroxidase conjugated goat anti human igg fab2
    Peroxidase Conjugated Goat Anti Human Igg Fab2, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peroxidase+conjugated+goat+anti+human+igg+fab2/Unconjugated+Goat+Anti-Human+IgG+Antibody/pm16322080-75-38-43
    Average 94 stars, based on 317 article reviews
    peroxidase conjugated goat anti human igg fab2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Plasma metallothionein antibody and cadmium-induced renal dysfunction in an occupational population in China.
    Article Snippet: Ninety-six-well microtiter plates (Corning Costar, Corning, NY, USA) were coated overnight with purified human fetus liver metallothionein (100 ll/well)—which was purified in our laboratory according to a previously described method (Nordberg et al., 1975b)—at 1 lg/ml in 0.05 mol/l carbonate buffer (pH 9.6) at 4 C. After plates were washed with phosphate-buffered saline (pH 7.4) containing 0.05% (v/v) Tween-20 (PBST), phosphate-buffered saline (200 ll/well) containing 10% (v/v) fetus bovine serum was added to both the human metallothionein-coated and non-coated plates, they were incubated for 1 h at 37 C to block any nonspecific binding. .. The plates were then washed with PBST, and the tested plasma (1:100; 100 ll/well) was added to both the metallothionein-coated and non-coated plates, which were then incubated for 1 h at 37 C. After five washes with PBST, peroxidase-conjugated goat anti-human IgG Fab2 (Vector Laboratories, Burlingame, CA, USA) was added (at 1:10, 000 dilution), and the plates were incubated for 30 min at 37 C. The plates were then washed five times with PBST and developed by the addition of orthophenylene diamine (Sigma, St. Louis, MO, USA) in the presence of 0.003% (v/v) H2O2. .. The enzyme reaction was stopped with 2 mmol/l H2SO4 (50 ll/well), followed by reading absorption at 492 nm (EIA reader Model ELX-800, Bio-Tek Instruments, Inc. Winooski, VT, USA).

    Incubation:

    Article Title: Plasma metallothionein antibody and cadmium-induced renal dysfunction in an occupational population in China.
    Article Snippet: Ninety-six-well microtiter plates (Corning Costar, Corning, NY, USA) were coated overnight with purified human fetus liver metallothionein (100 ll/well)—which was purified in our laboratory according to a previously described method (Nordberg et al., 1975b)—at 1 lg/ml in 0.05 mol/l carbonate buffer (pH 9.6) at 4 C. After plates were washed with phosphate-buffered saline (pH 7.4) containing 0.05% (v/v) Tween-20 (PBST), phosphate-buffered saline (200 ll/well) containing 10% (v/v) fetus bovine serum was added to both the human metallothionein-coated and non-coated plates, they were incubated for 1 h at 37 C to block any nonspecific binding. .. The plates were then washed with PBST, and the tested plasma (1:100; 100 ll/well) was added to both the metallothionein-coated and non-coated plates, which were then incubated for 1 h at 37 C. After five washes with PBST, peroxidase-conjugated goat anti-human IgG Fab2 (Vector Laboratories, Burlingame, CA, USA) was added (at 1:10, 000 dilution), and the plates were incubated for 30 min at 37 C. The plates were then washed five times with PBST and developed by the addition of orthophenylene diamine (Sigma, St. Louis, MO, USA) in the presence of 0.003% (v/v) H2O2. .. The enzyme reaction was stopped with 2 mmol/l H2SO4 (50 ll/well), followed by reading absorption at 492 nm (EIA reader Model ELX-800, Bio-Tek Instruments, Inc. Winooski, VT, USA).



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